I expect you will get a very high background--lots of false positives.  That's a problem with TUNEL and formalin fixed tissue anyway--formalin causes DNA strand breaks.
 
 

Jerry L. Ricks
Research Scientist
U.W. Medicine at South Lake Union
815 Mercer Street
Seattle, WA 98109
(206)-685-7190> Date: Fri, 31 Aug 2007 10:42:32 +0200> From: marijke.oste@ua.ac.be> To: histonet@lists.utsouthwestern.edu> Subject: [Histonet] TUNEL> > For staining the apoptotic cells I'm already using a kit of Roche> diagnostics. I think the greatest problem is that my tissues has been saved> for several years in ethanol 70%. Does anyone has experience in staining> tissues for apoptotic cells who are kept in ethanol for several years?> Thank you> > > _______________________________________________> Histonet mailing list> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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