RE: [Histonet] Fw: Thiocarbohydrazide

From:"Rittman, Barry R"

There is nothing wrong with your English.
Sorry that I do not have the protocol here.I believe that the point that John was trying to make and one with which I wholeheartedly concur is that this is a very dangerous chemical and you really need to not only have the protocol but understand the reaction and also exercise extreme care. This is for your safety and that of those also in your laboratory.
Most protocols do not provide adequete safety instructions for preparation of solutions.


From: on behalf of Agustin Chertcoff
Sent: Thu 8/23/2007 11:46 AM
Subject: Re: [Histonet] Fw: Thiocarbohydrazide

Hi histoneters!
Sorry, you maybe don't understand for my bad expression of the language.I don't ask so that  it is used. here are not books Pearse current. I will not invest money of my pocket for this stain. I need the simple protocol.  I  not wanted to buy a book,thanks!

I need this protocol,somebody that has made this reaction for the histochemical and ultracytochemical demonstration of glycomacromolecules for electron microscopy
TCH was introduced to cytochemistry, in the first adaptation,of the PAS reaction, to demonstrate tissue glycomacromolecules for TEM. The reaction depends upon selective oxidation of vicinalor 1,2-glycols, ethanolamines, a-hydroxyaldehydes, and ahydroxy-ketones.Enhancement of fine structure of cytomembranes by the osmium-thiocarbohydrazide-osmium (OTO) bridging. the OTO reaction as a simpler alternative to evaporative metal coating for SEM.

Excuse me if I didn't understand

thanks to all!

Ht Agustin Victor Chertcoff

National Institute of Microbiology.Electron Microscopy Service

Municipal Hospital of Gastroenterology

Patology Service.

 Buenos Aires.Argentina.South America

   John Kiernan
  To: Agustin Chertcoff
  Sent: Thursday, August 23, 2007 2:09 AM
  Subject: Re: [Histonet] Fw: Thiocarbohydrazide

  The answer to your question is simple.

  Buy a major histochemistry book and read it. That means the last edition of Pearse. All three volumes will cost about as much as 3 ml of of a rather dilute primary antibody that might turn out to be be worthless.

  Books by Hayat (in all biomedical libraries) also have much to say about thiocarbohydrazide. This is a field in which you must know what you are doing. Following a procedure that you do not understand will not deliver meaningful results.

  John Kiernan
  Anatomy, UWO
  London, Canada
  > ----- Original Message -----
  > From: Agustin Chertcoff
  > To:
  > Sent: Monday, August 13, 2007 5:47 PM
  > Subject: Thiocarbohydrazide
  > Hi Histoneters!
  > I need a protocole for OTO Technics (Osmium tetroxide,
  > Thiocarbohydrazide) for Electron Microcoscopy, specially as
  > preparing the Thiocarbohidrazide solution and the correct
  > temperature
  > Thanks in advanced !
  > Ht Agustin Victor Chertcoff
  > Electron Microscopy Service
  > National Institute of Microbiology C G Malbran
  > Buenos Aires
  > Argentina
  > _______________________________________________
  > Histonet mailing list
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