RE: [Histonet] Fw: Thiocarbohydrazide

From:"Smith, Allen"

Easier said than done: the 4th edition of Pearse's HISTOCHEMISTRY has been
out of print for years.  It took me 3 years of watchful waiting on Barnes
and Noble's used book site to get all 3 volumes.  Total cost was well over
It would be really nice if someone (Krieger? Dover?) reprinted it.

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
[] On Behalf Of John Kiernan
Sent: Thursday, August 23, 2007 1:10 AM
To: Agustin Chertcoff
Subject: Re: [Histonet] Fw: Thiocarbohydrazide

The answer to your question is simple.
Buy a major histochemistry book and read it. That means the last edition of
Pearse. All three volumes will cost about as much as 3 ml of of a rather
dilute primary antibody that might turn out to be be worthless. 
Books by Hayat (in all biomedical libraries) also have much to say about
thiocarbohydrazide. This is a field in which you must know what you are
doing. Following a procedure that you do not understand will not deliver
meaningful results.
John Kiernan
Anatomy, UWO
London, Canada
> ----- Original Message ----- 
> From: Agustin Chertcoff 
> To: 
> Sent: Monday, August 13, 2007 5:47 PM
> Subject: Thiocarbohydrazide
> Hi Histoneters!
> I need a protocole for OTO Technics (Osmium tetroxide, 
> Thiocarbohydrazide) for Electron Microcoscopy, specially as 
> preparing the Thiocarbohidrazide solution and the correct 
> temperature 
> Thanks in advanced !
> Ht Agustin Victor Chertcoff
> Electron Microscopy Service
> National Institute of Microbiology C G Malbran
> Buenos Aires
> Argentina 
> _______________________________________________
> Histonet mailing list
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