I've been working with some vibratome sections of brain and could use
some advice. I sectioned one brain and it came out ok. The blade
has a tendency to hang on the last bit of tissue and flip it over
producing and section with a slight thickening at the end. Despite
this I've gotten some good sections, but I'm having some problems
processing them. Is it possible to simply immerse some in fix? What
is the best way for handling this tissue? I'd like to take some and
dissect out the brain regions I'm interested in and use the rest for
histology. Currently the sections are 300 microns. Is this too
think to coverslip?
I've cut anther brain today and it went horribly, the brain just got
flattened, it seemed to get squished when the blade came along, I
could get a cut but it wasn't even, and I couldn't get any useful
sections. Any advice? Are there any online resources for how to
effectively use the vibratome for brains.
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