Hello Histonet
We hope next week to fix and mount some fluorescently vitally
stained bivalve (manila clams - (Rudi)Tapes philippinarum)
cleaving eggs and sperm.
For sperm alone, is it better to make a smear of live sperm
and fix on the slide or pre-fix? Should we use PDL coated
slides? APES? Subbed? SuperFrost Plus? What is the best fixative?
Likewise, any recommendations for attaching cleaving eggs to a
slide? Do shells need to be removed for pre-hatch stages? I am
also concerned that aldehyde fixatives might increase auto-
fluorescence of yolk etc. Any advice there?
There was a recent thread about resin mounting fluorescently
labeled materials. Any caveats about trying that with our
material?
Thanks, all!
hope you enjoy the long weekend!
-David
---------------
David A. Wright PhD
Biological Sciences Division
University of Chicago
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