Does anyone use a Thermo Shandon Excelsior tissue processor? We just
bought a new one, but found that the "state-of-the-art" molded polymer
chamber has small cracks at some of the seams, which would make reagent
containment and vacuum processing a bit difficult. Thermo is sending
someone to assess/replace the unit, but I want to know if anyone else
has experience with the machine to see if this is a common problem?
Has anyone ever seen cracks develop after repeated use? Any idea
whether the Shandon Pathcentre processor is better? Thanks!
-Jennifer Springsteen, Lab Manager
Lillehei Heart Institute Histopathology & Microscopy Core
University of Minnesota School of Medicine
Minneapolis, MN 55455
On Aug 17, 2007, at 11:10 AM, histonet-request@lists.utsouthwestern.edu
wrote:
> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
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>
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>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. detecting polyethylene glycol (Coskran, Timothy M)
> 2. Question about CLIA requirements (jimr0712@comcast.net)
> 3. Re: Question about CLIA requirements (Joe Nocito)
> 4. prostate needle biopsies (pereirafamily)
> 5. an easy solution RE: [Histonet] prostate needle biopsies
> (Cheryl R. Kerry)
> 6. Sensitivity and specificity (Laurie Reilly)
> 7. AW: [Histonet] Sensitivity and specificity (Gudrun Lang)
> 8. RE: Sensitivity and specificity (kemlo)
> 9. RE: detecting polyethylene glycol (kemlo)
> 10. Re: an easy solution RE: [Histonet] prostate needle biopsies
> (Rene J Buesa)
> 11. Chattanooga area.... (karenadams@comcast.net)
> 12. Ban on Formaldehyde in Europe deadline 22SEP07 (Jackie M
> O'Connor)
> 13. Xylene Substitute Testing Results (Rebecca Jones)
> 14. AW: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> (Gudrun Lang)
> 15. Re: Sensitivity and specificity (Rene J Buesa)
> 16. Re: Ban on Formaldehyde in Europe deadline 22SEP07 (Rene J Buesa)
> 17. RE: Sensitivity and specificity (kemlo)
> 18. RE: Ban on Formaldehyde in Europe deadline 22SEP07 (Edwards,
> R.E.)
> 19. RE: prostate needle biopsies (Cheri Miller)
> 20. wikipedia (Weston, Bernadette)
> 21. RE: Ban on Formaldehyde in Europe deadline 22SEP07
> (Bernice Frederick)
> 22. RE: {SPAM?} RE: [Histonet] prostate needle biopsies
> (Douglas D Deltour)
> 23. job postings (Deathridge, Mary Ann)
> 24. B&B Gram negative color change (Woodward, Denise)
> 25. Ban on Formaldehyde in Europe deadline 22SEP07 (Paula Pierce)
> 26. microwave reproducibility (GLORIA MUNOZ)
> 27. Re: B&B Gram negative color change (Rene J Buesa)
> 28. Re: Ban on Formaldehyde in Europe deadline 22SEP07 (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 16 Aug 2007 15:42:12 -0400
> From: "Coskran, Timothy M"
> Subject: [Histonet] detecting polyethylene glycol
> To:
> Message-ID:
> <3F4063B77CC1F445933078B93A0A0C720311D4D4@groamrexm05.amer.pfizer.com>
> Content-Type: text/plain; charset="US-ASCII"
>
> Does anyone know of a histochemical or IHC method for detecting
> polyethylene glycol in tissue samples?
>
>
>
> Thanks,
>
> Tim Coskran
>
> DSRD-Groton
>
> ----------------------------------------------------------------------
> LEGAL NOTICE
> Unless expressly stated otherwise, this message is confidential and
> may be privileged. It is intended for the addressee(s) only. Access
> to this E-mail by anyone else is unauthorized. If you are not an
> addressee, any disclosure or copying of the contents of this E-mail or =20
> any action taken (or not taken) in reliance on it is unauthorized and
> may be unlawful. If you are not an addressee, please inform the
> sender immediately.
>
> ------------------------------
>
> Message: 2
> Date: Thu, 16 Aug 2007 21:39:54 +0000
> From: jimr0712@comcast.net
> Subject: [Histonet] Question about CLIA requirements
> To: histonet@lists.utsouthwestern.edu (Histonet2),
> histonet-request@lists.utsouthwestern.edu (Histonet1),
> histonet-request@lists.utsouthwestern.edu (Histonet)
> Message-ID:
>
> <081620072139.27209.46C4C42A0007B15200006A492216554886CDCEC9CF9D030706@
> comcast.net>
>
>
> The IT staff at a hospital that we service, stated that CLIA requires
> the computer hardware to be under a CLIA license. Is this true ? Have
> not heard this before.
>
> Thanks.
>
> --
> Jim Robinson, M.S., HTL/HT (ASCP)
> Director of Laboratory Operations
> Orizon Diagnostics, LLC
> 102 Chestnut Avenue
> Westmont, Illinois 60559
> jrobinson@orizondiagnostics.com
> 630-321-1506 (fax)
> 630-230-6325
>
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 16 Aug 2007 17:30:12 -0500
> From: "Joe Nocito"
> Subject: Re: [Histonet] Question about CLIA requirements
> To: , "Histonet2"
> , "Histonet1"
>
> Message-ID: <000b01c7e055$03717e40$d49eae18@yourxhtr8hvc4p>
> Content-Type: text/plain; format=flowed; charset="Windows-1252";
> reply-type=original
>
> This is news to me and I just had a CLIA inspection last month.
>
> Joe
> ----- Original Message -----
> From:
> To: "Histonet2" ; "Histonet1"
> ; "Histonet"
>
> Sent: Thursday, August 16, 2007 4:39 PM
> Subject: [Histonet] Question about CLIA requirements
>
>
>> The IT staff at a hospital that we service, stated that CLIA requires =20
>> the
>> computer hardware to be under a CLIA license. Is this true ? Have not
>> heard this before.
>>
>> Thanks.
>>
>> --
>> Jim Robinson, M.S., HTL/HT (ASCP)
>> Director of Laboratory Operations
>> Orizon Diagnostics, LLC
>> 102 Chestnut Avenue
>> Westmont, Illinois 60559
>> jrobinson@orizondiagnostics.com
>> 630-321-1506 (fax)
>> 630-230-6325
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Thu, 16 Aug 2007 16:05:42 -0700
> From: "pereirafamily"
> Subject: [Histonet] prostate needle biopsies
> To:
> Message-ID: <000601c7e059$f8a40b40$09650744@pereirafarm>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Is anyone using anything other than sponges in their cassettes for
> prostate needle biopsies? If so, do you find that it works better?
>
> ------------------------------
>
> Message: 5
> Date: Thu, 16 Aug 2007 19:10:51 -0500
> From: "Cheryl R. Kerry"
> Subject: an easy solution RE: [Histonet] prostate needle biopsies
> To: "'pereirafamily'" ,
>
> Message-ID: <006a01c7e063$12e992f0$6701a8c0@CHERYLSLAPTOP>
> Content-Type: text/plain; charset="US-ASCII"
>
> You'll laugh--but this works GREAT. On top of the sponges a couple
> of labs
> I've worked at used white C-fold paper towels cut to size to lay
> against the
> biopsies. They process well, lift RIGHT OFF without sticking as long
> as the
> toweling was damp when the needle bxs were laid on them. No
> trebeculation
> from the sponges--and they were FLAT.
>
> It would layer like this:
>
> cassette,
> damp sponge,
> cut paper towel,
> biopsy(ies),
> cut paper towel,
> damp sponge,
> cassette lid.
>
> If you wanted to dye them squirt eosin or merchurichrome on the
> cassette
> once closed and they stain.
>
> Amazing-and pretty much free. It works with EMB and other 'sticky'
> stuff
> like FNA buttons.
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab, one great tech at a time.
> 800.756.3309
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> pereirafamily
> Sent: Thursday, August 16, 2007 6:06 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] prostate needle biopsies
>
> Is anyone using anything other than sponges in their cassettes for
> prostate
> needle biopsies? If so, do you find that it works better?
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 17 Aug 2007 17:05:49 +1000
> From: Laurie Reilly
> Subject: [Histonet] Sensitivity and specificity
> To:
> Message-ID: <002301c7e09d$0aa3e930$de55db89@health.ad.jcu.edu.au>
> Content-Type: text/plain; charset="us-ascii"
>
> Histonetters,
>
>
>
> Can anyone help with a concise definition of "sensitivity" and
> "specificity"
> as it relates to immunohistochemical reactions.
>
>
>
> Thanks and regards, Laurie.
>
>
>
> Mr. Laurie Reilly
>
> School of Veterinary & Biomedical Sciences
>
> James Cook University
>
> Townsville
>
> Queensland 4811
>
> Australia
>
>
>
> Phone 07 4781 4468
>
> Fax 07 4779 1526
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 17 Aug 2007 10:02:25 +0200
> From: "Gudrun Lang"
> Subject: AW: [Histonet] Sensitivity and specificity
> To: "'Laurie Reilly'"
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID: <000901c7e0a4$f360e360$6412a8c0@dielangs.at>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Sensitivity refers to the minimal ammount of antigen, that can be
> detected
> by the whole detection-system (primary antibody qualitiy/quantity;
> secondary
> antibody; activity of the used enzyme; etc.)
> The sensitivity can be encreased by higher affine antibodies, more
> "steps",
> amplification.
>
> The specifity of a test refers to the fact, that the test is only
> positiv,
> if the tested substance is really present; the test must not be
> positiv with
> any other substance. For example, if an antibody crossreacts with
> epitops on
> various celltypes, the antibody and the test are not specific for a
> unique
> cell-type. - but the antibody itself is specific to its epitop.
>
> Hope this helps
>
> Gudrun Lang
>
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
>
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von
> Laurie
> Reilly
> Gesendet: Freitag, 17. August 2007 09:06
> An: histonet@lists.utsouthwestern.edu
> Betreff: [Histonet] Sensitivity and specificity
>
> Histonetters,
>
>
>
> Can anyone help with a concise definition of "sensitivity" and
> "specificity"
> as it relates to immunohistochemical reactions.
>
>
>
> Thanks and regards, Laurie.
>
>
>
> Mr. Laurie Reilly
>
> School of Veterinary & Biomedical Sciences
>
> James Cook University
>
> Townsville
>
> Queensland 4811
>
> Australia
>
>
>
> Phone 07 4781 4468
>
> Fax 07 4779 1526
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 17 Aug 2007 11:21:27 +0100
> From: "kemlo"
> Subject: RE: [Histonet] Sensitivity and specificity
> To: "'Laurie Reilly'" ,
>
> Message-ID: <9453D8E8B8814677AA84C02BD8049AD9@KemloPC>
> Content-Type: text/plain; charset="us-ascii"
>
> Doesn't sensitivity mean that it will stain all of 'it', even that
> which
> isn't 'it'. Specificity means that it only stains 'it' but sometimes
> not all
> of 'it'.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie
> Reilly
> Sent: 17 August 2007 08:06
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Sensitivity and specificity
>
> Histonetters,
>
>
>
> Can anyone help with a concise definition of "sensitivity" and
> "specificity"
> as it relates to immunohistochemical reactions.
>
>
>
> Thanks and regards, Laurie.
>
>
>
> Mr. Laurie Reilly
>
> School of Veterinary & Biomedical Sciences
>
> James Cook University
>
> Townsville
>
> Queensland 4811
>
> Australia
>
>
>
> Phone 07 4781 4468
>
> Fax 07 4779 1526
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Fri, 17 Aug 2007 11:25:09 +0100
> From: "kemlo"
> Subject: RE: [Histonet] detecting polyethylene glycol
> To: "'Coskran, Timothy M'" ,
>
> Message-ID: <7F60092C0C774263B3EA2CD1855BFEC5@KemloPC>
> Content-Type: text/plain; charset="us-ascii"
>
> Won't polyethylene glycol wash out? You won't be able to process it so =20
> I
> guess it's frozen sections, but they won't freeze if there's
> polyethylene
> glycol present will they?
>
> Unless it gets broken down into a metabolite then I'm at a loss.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> Coskran,
> Timothy M
> Sent: 16 August 2007 20:42
> To: Histonet@pathology.swmed.edu
> Subject: [Histonet] detecting polyethylene glycol
>
> Does anyone know of a histochemical or IHC method for detecting
> polyethylene glycol in tissue samples?
>
>
>
> Thanks,
>
> Tim Coskran
>
> DSRD-Groton
>
> ----------------------------------------------------------------------
> LEGAL NOTICE
> Unless expressly stated otherwise, this message is confidential and
> may be
> privileged. It is intended for the addressee(s) only. Access to this
> E-mail by anyone else is unauthorized. If you are not an addressee,
> any
> disclosure or copying of the contents of this E-mail or any action
> taken (or
> not taken) in reliance on it is unauthorized and may be unlawful. If
> you
> are not an addressee, please inform the sender immediately.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 17 Aug 2007 06:10:51 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: an easy solution RE: [Histonet] prostate needle biopsies
> To: "Cheryl R. Kerry" , 'pereirafamily'
> , histonet@lists.utsouthwestern.edu
> Message-ID: <104128.57928.qm@web61215.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> You even could do WITHOUT the sponges, just the paper towel.
> René J.
>
> "Cheryl R. Kerry" wrote:
> You'll laugh--but this works GREAT. On top of the sponges a couple
> of labs
> I've worked at used white C-fold paper towels cut to size to lay
> against the
> biopsies. They process well, lift RIGHT OFF without sticking as long
> as the
> toweling was damp when the needle bxs were laid on them. No
> trebeculation
> from the sponges--and they were FLAT.
>
> It would layer like this:
>
> cassette,
> damp sponge,
> cut paper towel,
> biopsy(ies),
> cut paper towel,
> damp sponge,
> cassette lid.
>
> If you wanted to dye them squirt eosin or merchurichrome on the
> cassette
> once closed and they stain.
>
> Amazing-and pretty much free. It works with EMB and other 'sticky'
> stuff
> like FNA buttons.
>
> Cheryl Kerry, HT(ASCP)
> Full Staff Inc.
> Staffing the AP Lab, one great tech at a time.
> 800.756.3309
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> pereirafamily
> Sent: Thursday, August 16, 2007 6:06 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] prostate needle biopsies
>
> Is anyone using anything other than sponges in their cassettes for
> prostate
> needle biopsies? If so, do you find that it works better?
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ---------------------------------
> Park yourself in front of a world of choices in alternative vehicles.
> Visit the Yahoo! Auto Green Center.
>
> ------------------------------
>
> Message: 11
> Date: Fri, 17 Aug 2007 13:11:47 +0000
> From: karenadams@comcast.net
> Subject: [Histonet] Chattanooga area....
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
>
> <081720071311.28975.46C59E93000B65950000712F22070215539C030E0B0E020A9D0
> E05@comcast.net>
>
> Content-Type: text/plain
>
> Hello histonetters.....if anyone is on the server that works in or
> near the Chattanooga area could you please write me back.....Thank
> you!
>
> --
> Karen Adams
> Pathology Laboratories West
> 9303 Park West Blvd
> Knoxville, TN 37923
> (865) 690-2111 FAX (865) 691-1623
>
> ------------------------------
>
> Message: 12
> Date: Fri, 17 Aug 2007 08:19:55 -0500
> From: "Jackie M O'Connor"
> Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> To: histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Message-ID:
>
> Content-Type: text/plain; charset="US-ASCII"
>
> Funny thing - I found this information in Wikipedia.com and the related
> link below. After 22SEPT2007 it will be illegal to use formaldehyde =20
> as
> embalming fluid in Europe because of it's carcinogenic properties.
> Curious. Anyone in Europe have a comment?
> Jackie O'
>
>
> http://www.webwire.com/ViewPressRel.asp?aId=41468
>
> ------------------------------
>
> Message: 13
> Date: Fri, 17 Aug 2007 08:57:58 -0500
> From: "Rebecca Jones"
> Subject: [Histonet] Xylene Substitute Testing Results
> To: histonet
> Message-ID:
>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi all!
>
> Earlier this summer I asked many of you your opinion on a good xylene
> substitute to use. I got great suggestions and tips from all of you. =20
> I
> decided to test two, Clear Rite and Formula 83.
>
> Both performed well, but the Formula 83 has won the test. It
> performed most
> like xylene and required little to no changes in my procedures.
>
> I did choose Richard Allan Mounting Media, as it worked great with the
> Formula 83 and was suggested by the CBG rep. The mounting media I was
> using was not compatible with the sub.
>
> Formula 83 did dry slides slightly faster as they came off the stain
> line,
> but the slide quality still performed to the boss man's liking.
>
> Thank you all for your input. Back to work I go.
>
> Rebecca
>
>
> ------------------------------
>
> Message: 14
> Date: Fri, 17 Aug 2007 16:08:53 +0200
> From: "Gudrun Lang"
> Subject: AW: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> To: "'Jackie M O'Connor'"
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID: <000801c7e0d8$24e36ab0$6412a8c0@dielangs.at>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Embalming is no usual procedure in Austria. Perhaps that's the cause,
> why
> there wasn't any comment on this issue in the media. And the funeral
> homes
> are quiet institutions....
>
> Gudrun Lang
>
> Biomed. Analytikerin
> Histolabor
> Akh Linz
> Krankenhausstr. 9
> 4020 Linz
> +43(0)732/7806-6754
> -----Ursprüngliche Nachricht-----
> Von: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von
> Jackie M
> O'Connor
> Gesendet: Freitag, 17. August 2007 15:20
> An: histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu
> Betreff: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
>
> Funny thing - I found this information in Wikipedia.com and the related
> link below. After 22SEPT2007 it will be illegal to use formaldehyde =20
> as
> embalming fluid in Europe because of it's carcinogenic properties.
> Curious. Anyone in Europe have a comment?
> Jackie O'
>
>
> http://www.webwire.com/ViewPressRel.asp?aId=41468
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Fri, 17 Aug 2007 07:09:04 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] Sensitivity and specificity
> To: Laurie Reilly ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <269809.68833.qm@web61221.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Laurie:
> Both Gudrun and Kemlo are right, let me just try to put it in other
> way:
>
> 1-specificity: your detection system will react ONLY with the
> targeted component (it is, after all, an antigen/antibody reaction
> based on the quaternary protein structure of both), and
>
> 2-sensitivity: your detection system will detect ALL of the tageted
> component, even in its lowest concentrations. The more sensitive your
> detection system is, the less amount of the tageted compound it will
> be able to detect.
>
> René J.
>
> Laurie Reilly wrote:
> Histonetters,
>
>
>
> Can anyone help with a concise definition of "sensitivity" and
> "specificity"
> as it relates to immunohistochemical reactions.
>
>
>
> Thanks and regards, Laurie.
>
>
>
> Mr. Laurie Reilly
>
> School of Veterinary & Biomedical Sciences
>
> James Cook University
>
> Townsville
>
> Queensland 4811
>
> Australia
>
>
>
> Phone 07 4781 4468
>
> Fax 07 4779 1526
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Choose the right car based on your needs. Check out Yahoo! Autos new
> Car Finder tool.
>
> ------------------------------
>
> Message: 16
> Date: Fri, 17 Aug 2007 07:11:11 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> To: Jackie M O'Connor ,
> histonet@lists.utsouthwestern.edu,
> histonet-bounces@lists.utsouthwestern.edu
> Message-ID: <88243.841.qm@web61225.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Not from Europe, but we just should follow their example!
> René J.
>
> Jackie M O'Connor wrote: Funny thing - I =20
> found this information in Wikipedia.com and the related
> link below. After 22SEPT2007 it will be illegal to use formaldehyde as
> embalming fluid in Europe because of it's carcinogenic properties.
> Curious. Anyone in Europe have a comment?
> Jackie O'
>
>
> http://www.webwire.com/ViewPressRel.asp?aId=41468
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Got a little couch potato?
> Check out fun summer activities for kids.
>
> ------------------------------
>
> Message: 17
> Date: Fri, 17 Aug 2007 15:25:41 +0100
> From: "kemlo"
> Subject: RE: [Histonet] Sensitivity and specificity
> To: "'Rene J Buesa'" , "'Laurie Reilly'"
> ,
> Message-ID:
> Content-Type: text/plain; charset="iso-8859-1"
>
> I said it more succinctly .
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J =20
> Buesa
> Sent: 17 August 2007 15:09
> To: Laurie Reilly; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Sensitivity and specificity
>
> Laurie:
> Both Gudrun and Kemlo are right, let me just try to put it in other
> way:
>
> 1-specificity: your detection system will react ONLY with the
> targeted
> component (it is, after all, an antigen/antibody reaction based on the
> quaternary protein structure of both), and
>
> 2-sensitivity: your detection system will detect ALL of the tageted
> component, even in its lowest concentrations. The more sensitive your
> detection system is, the less amount of the tageted compound it will
> be able
> to detect.
>
> René J.
>
> Laurie Reilly wrote:
> Histonetters,
>
>
>
> Can anyone help with a concise definition of "sensitivity" and
> "specificity"
> as it relates to immunohistochemical reactions.
>
>
>
> Thanks and regards, Laurie.
>
>
>
> Mr. Laurie Reilly
>
> School of Veterinary & Biomedical Sciences
>
> James Cook University
>
> Townsville
>
> Queensland 4811
>
> Australia
>
>
>
> Phone 07 4781 4468
>
> Fax 07 4779 1526
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
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> _______________________________________________
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Fri, 17 Aug 2007 15:27:29 +0100
> From: "Edwards, R.E."
> Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> To: "Jackie M O'Connor" ,
> ,
>
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
> Apparently the recent accession of Romania to the EEC, which of
> course includes Transylvania has been the main stakeholders in
> this legislation.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie =20
> M
> O'Connor
> Sent: 17 August 2007 14:20
> To: histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu
> Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
>
> Funny thing - I found this information in Wikipedia.com and the related
> link below. After 22SEPT2007 it will be illegal to use formaldehyde
> as
> embalming fluid in Europe because of it's carcinogenic properties.
> Curious. Anyone in Europe have a comment?
> Jackie O'
>
>
> http://www.webwire.com/ViewPressRel.asp?aId=41468
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 19
> Date: Fri, 17 Aug 2007 09:47:46 -0500
> From: "Cheri Miller"
> Subject: RE: [Histonet] prostate needle biopsies
> To: "'pereirafamily'" ,
>
> Message-ID: <002c01c7e0dd$94e37f80$3402a8c0@plab.local>
> Content-Type: text/plain; charset="US-ASCII"
>
> We love the blue sponges.
>
>
> Cheri Miller HT ASCP
> Histology Supervisor
> Physicians Laboratory Services, Inc.
> Omaha, NE 68117
> 402 738 5052
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this
> message. If
> you are not the addressee intended / indicated or agent responsible for
> delivering it to the addressee, you are hereby notified that you are in
> possession of confidential and privileged information. Any
> dissemination,
> distribution, or copying of this e-mail is strictly prohibited. If
> you have
> received this message in error, please notify the sender immediately
> and
> delete this email from your system.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> pereirafamily
> Sent: Thursday, August 16, 2007 6:06 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] prostate needle biopsies
>
> Is anyone using anything other than sponges in their cassettes for
> prostate
> needle biopsies? If so, do you find that it works better?
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this
> message. If
> you are not the addressee intended / indicated or agent responsible for
> delivering it to the addressee, you are hereby notified that you are in
> possession of confidential and privileged information. Any
> dissemination,
> distribution, or copying of this e-mail is strictly prohibited. If
> you have
> received this message in error, please notify the sender immediately
> and
> delete this email from your system.
>
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this
> message. If you are not the addressee intended / indicated or agent
> responsible for delivering it to the addressee, you are hereby
> notified that you are in possession of confidential and privileged
> information. Any dissemination, distribution, or copying of this
> e-mail is strictly prohibited. If you have received this message in
> error, please notify the sender immediately and delete this email from =20
> your system.
>
>
>
>
> ------------------------------
>
> Message: 20
> Date: Fri, 17 Aug 2007 10:01:18 -0500
> From: "Weston, Bernadette"
> Subject: [Histonet] wikipedia
> To:
> Message-ID:
>
> <5213EA72AB13584EBD29CE54E1A66F0502954233@CPRTEVS02.triadhospitals.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Not to cast doubt but are we sure Wikipedia has got this right? They
> have been called questioned recently.
>
> Bernadette Weston HT
> Histology Supervisor
> Barberton Citizens Hospital
> 330 615 3980
> bweston@barbhosp.com
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Fri, 17 Aug 2007 10:04:43 -0500
> From: "Bernice Frederick"
> Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> To: "'Edwards, R.E.'" , "'Jackie M O'Connor'"
> , ,
>
> Message-ID: <000001c7e0df$f4ba3000$d00f7ca5@lurie.northwestern.edu>
> Content-Type: text/plain; charset="us-ascii"
>
> You mean there is a clone of Joe in the U.K.? Love it!!!!
>
> Bernice Frederick HTL (ASCP)
> Northwestern University
> Pathology Core Facility
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> Edwards,
> R.E.
> Sent: Friday, August 17, 2007 9:27 AM
> To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu
> Subject: RE: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
>
> Apparently the recent accession of Romania to the EEC, which of
> course includes Transylvania has been the main stakeholders in
> this legislation.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie =20
> M
> O'Connor
> Sent: 17 August 2007 14:20
> To: histonet@lists.utsouthwestern.edu;
> histonet-bounces@lists.utsouthwestern.edu
> Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
>
> Funny thing - I found this information in Wikipedia.com and the related
> link below. After 22SEPT2007 it will be illegal to use formaldehyde
> as
> embalming fluid in Europe because of it's carcinogenic properties.
> Curious. Anyone in Europe have a comment?
> Jackie O'
>
>
> http://www.webwire.com/ViewPressRel.asp?aId=41468
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 22
> Date: Fri, 17 Aug 2007 11:09:54 -0500
> From: "Douglas D Deltour"
> Subject: RE: {SPAM?} RE: [Histonet] prostate needle biopsies
> To: "'Cheri Miller'" , "'pereirafamily'"
> ,
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
> Ask your processor if it loves the blue sponges. :)
>
> Douglas D. Deltour HT(ASCP)
> Histology Manager
> Professional Pathology Services, PC
> One Science Court
> Suite 200
> Columbia, SC 29203
> Office (803)252-1913
> Fax (803)254-3262
> Doug@ppspath.com
> *****************************************************
> PROFESSIONAL PATHOLOGY SERVICES, PC
> NOTICE OF CONFIDENTIALITY
> This message is intended only for the use of the individual or entity
> to
> which it is addressed and may contain information that is privileged,
> confidential and exempt from disclosure under applicable law. If the
> reader
> of this message is not the intended recipient, you are hereby notified =20
> that
> any dissemination, distribution, or copying of this communication is
> strictly prohibited by law. If you have received this communication in
> error, please notify me immediately.
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheri
> Miller
> Sent: Friday, August 17, 2007 9:48 AM
> To: 'pereirafamily'; histonet@lists.utsouthwestern.edu
> Subject: {SPAM?} RE: [Histonet] prostate needle biopsies
>
> We love the blue sponges.
>
>
> Cheri Miller HT ASCP
> Histology Supervisor
> Physicians Laboratory Services, Inc.
> Omaha, NE 68117
> 402 738 5052
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this
> message. If
> you are not the addressee intended / indicated or agent responsible for
> delivering it to the addressee, you are hereby notified that you are in
> possession of confidential and privileged information. Any
> dissemination,
> distribution, or copying of this e-mail is strictly prohibited. If
> you have
> received this message in error, please notify the sender immediately
> and
> delete this email from your system.
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
> pereirafamily
> Sent: Thursday, August 16, 2007 6:06 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] prostate needle biopsies
>
> Is anyone using anything other than sponges in their cassettes for
> prostate
> needle biopsies? If so, do you find that it works better?
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this
> message. If
> you are not the addressee intended / indicated or agent responsible for
> delivering it to the addressee, you are hereby notified that you are in
> possession of confidential and privileged information. Any
> dissemination,
> distribution, or copying of this e-mail is strictly prohibited. If
> you have
> received this message in error, please notify the sender immediately
> and
> delete this email from your system.
>
>
> PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this
> message. If
> you are not the addressee intended / indicated or agent responsible for
> delivering it to the addressee, you are hereby notified that you are in
> possession of confidential and privileged information. Any
> dissemination,
> distribution, or copying of this e-mail is strictly prohibited. If
> you have
> received this message in error, please notify the sender immediately
> and
> delete this email from your system.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
> ------------------------------
>
> Message: 23
> Date: Fri, 17 Aug 2007 10:12:39 -0500
> From: "Deathridge, Mary Ann"
> Subject: [Histonet] job postings
> To:
> Message-ID:
> <113162616B6440479A211712490222C684C4CA@mailbe10.mc.vanderbilt.edu>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Vanderbilt University Medical Center, Nashville, TN. has (2) two
> histotechnologist (ASCP) position openings in the histopathology lab. =20
> We are looking for techs with immunohistochemistry experience, as well =20
> as, general histology skills.
> VUMC offers a great benefits package and a highly competitive salary
> range in line with today's market.
> Vanderbilt prides itself in being one of the best places to work with
> high employee satisfaction.
> Check out the web site at www.mc.vanderbilt.edu
> go to HR, available jobs, to post
> resume.
> Feel free to contact me for further information.
>
> MaryAnn Deathridge, BS., HT (ASCP)
> Supervisor, Histopathology
> (615) 343-7012
>
>
> ------------------------------
>
> Message: 24
> Date: Fri, 17 Aug 2007 11:26:11 -0400
> From: "Woodward, Denise"
> Subject: [Histonet] B&B Gram negative color change
> To: "Weston, Bernadette" ,
>
> Message-ID:
> <40AC6D73C2B95C4CA21B26B7BF380C401662FC@EXCHANGED.mgmt.ad.uconn.edu>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Hello all,
>
> We recently noticed that our B&B positive controls change color over
> time after staining. It happens in as little time as a week.
> The Gram negative organisms change hue from a nice pink/red to a purple
> color. The Gram positive organisms remain blue.
> Anyone have any clues as to why/how this is happening?
> Sorry to ask a difficult question on a Friday but this one has been
> bothering me for a few days.
> Thanks,
> Denise Long Woodward
> UConn
> Dept. of Pathobiology and Veterinary Sciences
> Storrs, CT
>
>
>
> ------------------------------
>
> Message: 25
> Date: Fri, 17 Aug 2007 08:28:13 -0700 (PDT)
> From: Paula Pierce
> Subject: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> To: Histonet
> Message-ID: <171741.71965.qm@web50111.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> So, the "undead" of Transylvania have a large enough lobby to get all
> of Europe against embalming with formaldehyde? ;)
>
>
> In all honesty though, beware the slippery slope of banning something
> for one use. Our astronaut's safety has become secondary to using
> inferior materials for environmental purposes.
>
>
> Paula Pierce, HTL(ASCP)HT
>
> ------------------------------
>
> Message: 26
> Date: Fri, 17 Aug 2007 08:36:50 -0700 (PDT)
> From: GLORIA MUNOZ
> Subject: [Histonet] microwave reproducibility
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <975935.62550.qm@web80206.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi HistoNetters:
> Just wondering if any of you have a procedure to share on
> microwave reproducibility for a store-bought, non-medical microwave.
> We had our CAP inspection recently and were asked to come up with a
> method to test the microwave. The inspector recommended heating
> distilled water for one minute at each of the three different power
> levels and documenting the results. Does anyone know of another,
> maybe better, way?
> Thanks a lot, enjoy reading your entries, and hope you all have a =20
> restful weekend...........gloria
>
>
> ------------------------------
>
> Message: 27
> Date: Fri, 17 Aug 2007 08:55:41 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] B&B Gram negative color change
> To: "Woodward, Denise" , "Weston,
> Bernadette" , histonet@lists.utsouthwestern.edu
> Message-ID: <60752.31377.qm@web61217.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> If you do not totally wash/remove/differentiate between steps, that
> could happen.
> René J.
>
> "Woodward, Denise" wrote:
>
> Hello all,
>
> We recently noticed that our B&B positive controls change color over
> time after staining. It happens in as little time as a week.
> The Gram negative organisms change hue from a nice pink/red to a purple
> color. The Gram positive organisms remain blue.
> Anyone have any clues as to why/how this is happening?
> Sorry to ask a difficult question on a Friday but this one has been
> bothering me for a few days.
> Thanks,
> Denise Long Woodward
> UConn
> Dept. of Pathobiology and Veterinary Sciences
> Storrs, CT
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Need a vacation? Get great deals to amazing places on Yahoo! Travel.
>
> ------------------------------
>
> Message: 28
> Date: Fri, 17 Aug 2007 08:57:12 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] Ban on Formaldehyde in Europe deadline 22SEP07
> To: Paula Pierce , Histonet
>
> Message-ID: <839201.66477.qm@web61216.mail.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Remember how the Transilvanian "undeads"increase in numer by neck
> bitting/blood sucking.
> René J.
>
> Paula Pierce wrote:
> So, the "undead" of Transylvania have a large enough lobby to get
> all of Europe against embalming with formaldehyde? ;)
>
>
> In all honesty though, beware the slippery slope of banning something
> for one use. Our astronaut's safety has become secondary to using
> inferior materials for environmental purposes.
>
>
> Paula Pierce, HTL(ASCP)HT
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ---------------------------------
> Luggage? GPS? Comic books?
> Check out fitting gifts for grads at Yahoo! Search.
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 45, Issue 24
> ****************************************
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