We use it and have used it for some time. It seems to work fairly well,
though none of the samples we do are human patients. We are exclusively a
research lab. The nice thing is that it doesn't seem to require antigen retrieval
to the extent of formalin-fixed tissues.
However, in light of samples I have worked with lately, I am finding that
some IHC doesn't seem to work as well after long-term storage (~5 years).
As to staining (H&E), there seems to be very little difference in intensity
and has not affected our IHC.
All that said, I have not done a side-by-side comparison on the same tissues
fixed with Histochoice vs Formalin, though I do have some in the wings to do
this eventually.
Albert
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation
Tissue Engineering Lab, Saint Patrick Hospital
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