[Histonet] Re:Histochoice


We use it and have used it for some time.  It seems to work fairly  well, 
though none of the samples we do are human patients.  We are  exclusively a 
research lab.  The nice thing is that it doesn't  seem to require antigen retrieval 
to the extent of formalin-fixed tissues.
However, in light of samples I have worked with lately, I am finding  that 
some IHC doesn't seem to work as well after long-term storage (~5  years).
As to staining (H&E), there seems to be very little difference  in intensity 
and has not affected our IHC. 
All that said, I have not done a side-by-side comparison on the same  tissues 
fixed with Histochoice vs Formalin, though I do have some in the wings  to do 
this eventually.
Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital

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