Igor,
Use mouse anti-human alpha-smooth muscle actin, clone IA4, available from 
Sigma, Labvision, and probably everybody else. Works at 1:1000 for 
immunofluorescence and also works for IHC. It is currently the one and 
only marker to differentiate the two cell types from each other. I used 
the Sigma antibody in this publication:

=0AVaughan, M.B., Howard, E.W., and Tomasek, J.J. (2000). Transforming growth 
factor-ß1 promotes the morphological and functional differentiation of the 
myofibroblast. Exp. Cell Res. 257:180-189. 

Mel

Melville B. Vaughan, Ph. D.
Assistant Professor of Biology
Campus Coordinator, Sure-Step program
=0AUniversity of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm

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