[Histonet] ISH

From:"Margaryan, Naira"



Hi histonetters,

 

I got a protocol for ISH for cells and there is a step, which looks very
strange for me: After cytospin cells on glass slides let them air dry at
RT for 60min. Is it not too long for the 60 min drying? Will cells
remain on the glass in this case or there is a risk pop off cells from
the glass?

 

Thanks in advance,

Naira

 

Naira V. Margaryan, D.V.M., Ph.D.

Children's Memorial Research Center

2300 Children's Plaza, Box 222

Chicago, IL 60614-3394

Tel: 773-755-6570/ext-8

Fax: 773-755-6594

nmargaryan@childrensmemorial.org
 

 

For Express Mail: 

CMRC, Room C.473

2430 N. Halsted Street

Chicago, IL  60614-4314

 

 

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