We carry out IF experiments routinely and never dehydrate, clear and
mount the slides.
One we had to clear IF slides for Laser Capture Micro dissection and
after clearing some of the slides lost the signal. I wanted to see if
it is the alcohols or the xylene.
After testing: some of the slides lost the signal after dehydration
and some after xylene. It was depending not only on the primary but
also on the secondary antibody.
New York, NY 10021
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