I have never used urea in nitric acid, but simply 5% nitric acid, any
higher concentration is a bit too concentrated. However, if the stock
nitric acid has a yellow color to start with, DON'T use it. The yellow
color means the acid is old and you should use new stock instead. Plus you
may never get rid of the yellow discoloration.
There is a nitric acid decalcifier caller Perenyi's (1882!) that gave us
wonderful results years ago in a study, and is a bit gentler than 5% nitric
Nitric acid, 10% 40 ml
Absolute ethanol 30 ml
0.5% chromic acid 30 ml
We did decalcification endpoint determinations with this decalcifier, and
had brilliant staining results.
At 09:21 AM 8/6/2007, you wrote:
>Hi - For those of you who decalcify a lot. I have a graduate student who
>want to decalcify temporal bone from a dog using a protocol that uses 5%
>nitric acid and urea. The urea is supposed to clear the nitric acid color.
> Our question is - is there a specific urea what should be used. Having
>never used it myself, I have no idea what to buy.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
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