Oh well, nevermind! Thanks Gayle...
[mailto:firstname.lastname@example.org] On Behalf Of Gayle
Sent: Wednesday, August 08, 2007 3:24 PM
Subject: [Histonet] Hales Colloidal iron
You should also look in the Histonet archives for this, there were
discussions in 1999, 2002 and 2003.
The Muller/Mowry's or Mowry's modification of Hales should work, and is
found in Frieda Carsons book.
This was posted on Histonet in 2002 and found in Histonet Archives
According to Pearse's Histochemistry: Theoretical and applied, Hales
colloidal iron is as follows:
Fix in Wolman, Carnoy or formalin. Paraffin sections. Avoid adhesives.
1. Sections to water.
2. Mix 1 vol dialysed iron and 1 vol 2M acetic acid. Apply for 10
3. Wash very well with distilled water to remove all traces of iron.
4. Do Perls' prussian blue.
5. Wash well with water.
6. Counterstain with neutral red 1 min.
7. Dehydrate, clear, mount
Acid mucins - blue
Nuclei - red
Rinehart and Abu'l Haj dialysed iron
75 g ferric chloride
250 mL water
Dissolve and add 100 mL glycerol.
While stirring, add 55 mL strong ammonia.
Dialyse against distilled water for three days, changing the water
This mixture triples in volume by the end of dialysis.
----- Original Message -----
From: "Goodwin, Diana"
Sent: Thursday, April 04, 2002 8:16 AM
Subject: Hale's method for colloidal iron
> Greetings, Netters.>
> Does anyone have a copy of Hale's method for colloidal iron? My
pathologist has asked for it specifically, probably because it is cited
literature for differentiation of chromophobe RCC.
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
Histonet mailing list
Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc.
Histonet mailing list