RE: [Histonet] FISHing trip

From:"Tarango, Mark"

I don't have a protocol for doing this on urine, but if I wanted to do
it, the first thing I'd try would be trypsanizing the cells after
spinning to obtain a pellet.  Then I'd spin down again, decant the
remaining trypsan, re-suspend in PBS, and make cytospins.  Then it's
happy FISHing!

Mark Adam Tarango HT(ASCP)

Histology & IHC Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

Direct Line (702) 822-5112

Mobile (702) 759-9229

Fax (702) 939-7663


-----Original Message-----
[] On Behalf Of Lester
Sent: Monday, August 13, 2007 10:34 AM
Subject: [Histonet] FISHing trip

I haven't gotten any help from the pathologist listserve, and the
UroVysion FISH listserve doesn't have many users, so I am asking the
histotechs for help!  Maybe you have friends doing FISH....



We are doing FISH studies on urine (UroVysion) and frequently run into
cell clumps/clusters.  Since the architecture is not what we are
assessing via FISH, it would be useful to disrupt these clumps and
obtain single cells for analysis.  Does anyone have a protocol for doing
this on urine specimens?


Thanks in advance.



Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, IL 60154

ph:  708-486-0076
fax: 708-486-0080


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