RE: [Histonet] FISHing trip

From:"Tarango, Mark"



I don't have a protocol for doing this on urine, but if I wanted to do
it, the first thing I'd try would be trypsanizing the cells after
spinning to obtain a pellet.  Then I'd spin down again, decant the
remaining trypsan, re-suspend in PBS, and make cytospins.  Then it's
happy FISHing!


Mark Adam Tarango HT(ASCP)

Histology & IHC Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

Direct Line (702) 822-5112

Mobile (702) 759-9229

Fax (702) 939-7663

  


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester
Raff
Sent: Monday, August 13, 2007 10:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FISHing trip

I haven't gotten any help from the pathologist listserve, and the
UroVysion FISH listserve doesn't have many users, so I am asking the
histotechs for help!  Maybe you have friends doing FISH....

 

 

We are doing FISH studies on urine (UroVysion) and frequently run into
cell clumps/clusters.  Since the architecture is not what we are
assessing via FISH, it would be useful to disrupt these clumps and
obtain single cells for analysis.  Does anyone have a protocol for doing
this on urine specimens?

 

Thanks in advance.

 

 

Lester J. Raff, MD
Medical Director
UroPartners, LLC Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, IL 60154


ph:  708-486-0076
fax: 708-486-0080

 

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


"EMF " made the following annotations.
------------------------------------------------------------------------------
CONFIDENTIALITY NOTICE: This e-mail message, including any
attachments, is for the sole use of the intended 
recipient(s) and may contain confidential, proprietary, 
and/or privileged information protected by law. If you are 
not the intended recipient, you may not use, copy, or 
distribute this e-mail message or its attachments. If you 
believe you have received this e-mail message in error, 
please contact the sender by reply e-mail and destroy all 
copies of the original message
==========================3D=========================3D=========================3D==


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




<< Previous Message | Next Message >>