Hi to all fixation-experts,
I've found in the literature some controversy statements about
formaldehyd-fixation and want your help, what is the up-do-date-knowledge.
- Formaldehyd can/cannot be washed out of fixed tissue.
- Formaldehyd fixates slower in more acid pH; uncharched
aminogroups react better than protonated with formaldehyd. And these groups
should be uncharged in neutral pH.
- Nuclear chromatin is fixed only by acid fixatives.
What is your opinion on these statements?