I'm writing because I have problem with de-staining of my samples during the staining with crysel violet. I read from the message from 2003 that the way is to dry samples in the oven, and don't use the water and alcoholes for dehydratation and differentiation. I tried to add acetic acid to alcoholes, to avoid 70, 95%...And nothing. The sections are a little bit blue after even 12 mins in crysel violet an than they are destained. I tried it with different samples, from formalin, Bouin's fixative. Finnaly, I have to try with drying in the oven but now I try to find other resolution.
Crysel violet is from Sigma-Aldrich.
I will appreciate for your help.
Thanks
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet