Hi Histoneters!
I need a protocole for OTO Technics (Osmium tetroxide, Thiocarbohydrazide)specially as preparing the hidrocarbothyazide solution and the correct temperature 

Thanks in advanced !

Ht Agustin Victor Chertcoff
Electron Microscopy Service
National Institute of Microbiology C G Malbran
Buenos Aires
Argentina 
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