As most of the people posting a message here...I need help!
One user is trying to detect aromatase by immunofluorescence in mouse
ovary....problem: the autofluorescence on the cells around the follicle is
too high and we still didn't get any specific staining or maybe the staining
is weaker than the autofluorescence. The fact is that the autofluorescence
is really high only in these cells and not all over the tissue!
The tissue is frozen and PFA fixed. We also tried to use Far Red secondary
antibody but with no luck.
Any suggestion will be great
Berno Valeria, PhD
EMBL Monterotondo Outstation
via Ramarini 32
00015 Monterotondo Scalo (RM)
Tel: +39 06 90091 287
Fax: +39 06 90091 272
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