I am in need of your input once again!
The researchers here use a product called MI Embedding Matrix,
manufactured by Thermo Shandon, (or whatever name they use now) or have
knowledge of it. The researchers here embed their own frozens and I cut
them. Peridoically over the years we see "holes" where the nuclei
should be. I have contacted Thermo Shandon for their tech support, and
followed everything they suggested. I am at a loss.
The tissue being embedded is in vitro skin substitute with human cells;
very small and very thin, so I don't think this is a big issue.
I cut at 4 - 5 microns at -20. The only thing affected is the nucleus.
I am really puzzled and do not really know where to turn for answers.
Any thoughts are appreciated.
Thanks in advance,
Deanna Snider HT ASCP
Shriners Hospital for Children
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