I am cutting adult rat brainstems, transcardially perfused with 4%
paraformaldehyde in PBS and immersed in same overnight at 4c then sunk in 30
sucrose in PBS cutting at 8 microns on cryostat as they dry they rip
regards of the type of slide. What in the world am I doing wrong been
cutting for 25 yr. and this is a first for me. Box at -25c using high
profile accu-edge blade, using both OCT and TBS no help still ripping
Took sample in paraffin good cut at 8 microns
Sr. Res. Tech. Histology Core Manager
Washington University School of Medicine
Department of Otolaryngology
Campus box 8115
4566 Scott Ave.
St. Louis, MO. 63110
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