[Histonet] Cryo sections

From:Pat Keller

I am cutting adult rat brainstems, transcardially perfused with 4%
paraformaldehyde in PBS and immersed in same overnight at 4c then sunk in 30
sucrose in PBS cutting at 8 microns on cryostat  as they dry they rip
regards of the type of slide. What in the world am I doing wrong been
cutting for 25 yr. and this is a first for me. Box at -25c  using high
profile accu-edge blade, using both OCT and TBS no help still ripping
Took sample in paraffin good cut at 8 microns

Pat Keller

Patricia Keller, 
Sr. Res. Tech.   Histology Core Manager
Washington University School of Medicine
Department of Otolaryngology
Campus box 8115
4566 Scott Ave.
St. Louis,   MO.   63110

OFFICE    314-747-7166
FAX          314-747-7230

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