Hello All
 
Has anyone out there worked on IHC staining of different varients of CD44 on human brain.  I had what I thought was really nice staining but the client thinks that all I am staining is lipofucsin.  My negative controls are negative and the areas that are staining do not autofluoresce, I read some where that lipofuchsin autofluoresces so I'm a bit confused.  I do have images available if anyone would like to review them.  The antibodies are from Chemicon.  Any help would be appreciated. 
 
Thanks in advance
 
Liz
 
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
HYPERLINK "mailto:liz@premierlab.com"liz@premierlab.com
HYPERLINK "http://www.premierlab.com/"www.premierlab.com
 
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Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309
 

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