I have found the statements in Luna's "Histopathologic methods and color
atlas of special stains and tissue artefacts" (1992) in the chapter
Formerly I have read your book, which impressed me very much. And after
learning much about the staining-theory and pH, I thought Luna's statements
were wrong or at least not understandable for me.
What do you think?
Von: John Kiernan [mailto:firstname.lastname@example.org]
Gesendet: Dienstag, 07. August 2007 08:00
Betreff: Re: [Histonet] fixation-question
Have you read and compared the statements you claim to have found "in the
----- Original Message -----
From: Gudrun Lang
Date: Monday, August 6, 2007 14:19
Subject: [Histonet] fixation-question
> Hi to all fixation-experts,
> I've found in the literature some controversy statements about
> formaldehyd-fixation and want your help, what is the up-do-date-
> Formaldehyd can/cannot be washed out of fixed tissue.
> Formaldehyd fixates slower in more acid pH; uncharched
> aminogroups react better than protonated with formaldehyd. And
> these groups
> should be uncharged in neutral pH.
> - Nuclear
> chromatin is fixed only by acid fixatives.
> What is your opinion on these statements?
> Gudrun Lang