Re: [Histonet] rat femur histopath

From:Gayle Callis


Any acid decalcifier should work fine, we use 10% formic acid but you need 
to do endpoint determinations to make sure the bone and soft tissues are 
not ruined by the effects of overexposure to acid aka 
overdecalcification.   Rinse the bones for a few hours, process longer into 
paraffin unless you are bisecting the femurs.  We did our rat femurs for 2 
hours per station on a VIP, and use Tissue Prep 2, a harder paraffin that 
is better for sectioning for rat femurs and whole rat knees.

I have a quick weight loss/weight gain endpoint determination that works 
well if you do not have a FAXITRON to xray for endpoint, or do not want to 
do a chemical endpoint test - you can do that too.

At 12:57 PM 8/29/2006, you wrote:
>Hi all,
>I am looking for a procedure for delcalcifying rat femur.  I want to
>visualize and quantify BM fibrosis using Gomori stain.  If anyone has a good
>protocol please let me know.
>Thank you
>Melissa Aycock
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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