Re: [Histonet] Small endoscopy specimens

From:"sheila adey"

Do you use mineral oil on your processor for large specimens to? I would 
like to see your protocol aswell. We have on occasion had this same problem.
Sheila HT, MLT
Port Huron Hospital

>From: Rene J Buesa 
>To: Patricia Karlisch , 
>Subject: Re: [Histonet] Small endoscopy specimens
>Date: Tue, 29 Aug 2006 09:53:12 -0700 (PDT)
>   You yourself have pointed out to the 2 main sources of the problem:
>   1- a longer than needed processing schedule is one. Reducing it, 
>specially the dehydrating stations, can help, and
>   2- doing a better coordination between the OR and the lab (with regards 
>to how expeditiously the Bx are fixed/delivered).
>   In our case we did the same two things but we really eliminated the 
>problem when we eliminated xylene in our tissue processing and changed to 
>mineral oil.
>   If you want I can send you that protocol.
>   Regards
>   René J.
>Patricia Karlisch  wrote:
>   All,
>We are having occassional problems with small colon, stomach and
>antrum specimens that are 'drying' out, at least this is what we think.
>The specimens show no nuclear detail and the H&E looks smudgy. Other
>specimens stained with the same slides look great. We process our small
>specimens on a short 5 hour processing schedule which seemed to have
>improved some of our 85% of our specimens. Has anyone else had this
>problem? We think that the specimen may be sitting in the OR before
>they get placed in formalin. Is there any way to fix this when it
>happens? Any suggestions would be helpful. Pat Karlisch
>Pat Karlisch
>Supervisor, Histology, Pathology and Laboratory Medicine
>Penn State Milton S. Hershey Medical Center
>Mail Code H179
>Hershey, PA 17033
>Phone (717) 531-6072
>Fax: (717) 531- 7741
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