Re: [Histonet] OCT embedded Tissue Storage

From:Rene J Buesa

Mathew:
  In my lab we used to have a "tissue back" for teaching/reference purposes.
  All tissues were kep in OCT at -80║C in a freezer, and we used to have tissues more than 15 years old.
  When needed, they were FS without any problem.
  RenÚ J.

Mathew DeGutes  wrote:
  I have a couple questions. In the lab I work in we have started to accumulate a number of
experiments worth of tissue blocks that we would like to keep for reference at later dates.
Generally tissue survives better while still embedded in a block than when in slide form, so I was
curious as to what sorts of methods others here employ to keep order and good preservation of
blocks in -80s? Up until now we have been just keeping individual sets of blocks in Ziplock bags,
but it has gotten to be unwieldy. I am looking for any suggestions of a good way to keep the
blocks well preserved and easily recovered in -80s.



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