This has been answered on Histonet several times, just go to Archives and 
type in F4/80.  Barb Wright is one who has given good info on using this 
antibody from Serotec.  There will be other excellent replies also.

At 02:18 AM 8/24/2006, you wrote:

>   I would be interested in hearing from anyone who has used Sertec F4/80
>   to  do  IHC  for   microglia  in pfa fixed mouse brains. I have lovely
>   staining  in  my  kupfer  cells  (control)  but  only staining of what
>   appears  to  be  neurones  in  the  brain. A protocol would be greatly
>   appreciated.
>
>   Thanks in advance
>
>   Helen
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Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)



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