RE: [Histonet] Re: Immunostaining for H. pylori

From:"sheila adey"

We have been using the AEC polymer detectiong kit for the Biogenex i6000 and 
find good staining with that.
Can anyone tell me if they have any experience with the Biocare Nemesis 
7200, they say that they offer true random access? Not just continuous 
access? Any comments would be greatly appreciated

>Subject: [Histonet] Re: Immunostaining for H. pylori
>Date: Wed, 16 Aug 2006 13:24:36 EDT
>Joseph Nerk (where?) asks:
> >>Does any in histo land can recommend a good Immunostaining detection kit
>for Helicobacter pylori, have been seeing a lit recently but the 
>looks at the Giemsa but still will like the Immunostaining method.<<
>For the last year or so we've been using the Cell Marque polyclonal, with a
>Ventana stainer, and have had very little trouble with it. As a 
>pathologist, my
>take on immunostaining Helicobacter is that it saves me a lot of time. With
>one stain a day and 20 cases to sign out, I don't mind searching a Giemsa 
>Diff-Quik II stain with an oil immersion lens, but if I've got 10 cases a 
>and 70 cases to sign out, it's a great help.
>Another point to consider: if you do the immunostain, you greatly increase
>the total number of immunostains you do. That can justify setting up
>immunohistochemistry in a pathology service that's otherwise too small to 
>have it.
>Bob Richmond
>Gastonia NC
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