Yes, five minutes in each xylene is our protocol and we use 3 xylenes.   Janet

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of sheila adey
Sent: Thu 8/31/2006 10:39 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Deparaffinization question



Hello Histonetters. 

I am curious about how long other labs are spending in Xylene when bringing 
tissue to water? I started here 6 years ago and never questioned the 
rehydration times but I think we may not be spending enough time in Xylene. 
Our times are 1 min in each of 2 xylenes. This week we increased it to 5 
minutes in each and I think our IHCs are staining better? 
Any input? 
Thanks 

Sheila Adey HT, MLT 
Port Huron Michigan 

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