[Histonet] question about sirius red stain

From:"Xilong Li"

Hi, All,

I stain collagen of frozen muscle section using regular sirius red
staining protocol(0.5g sirius red F3B in 500ml saturated picric acid,
section were stained 1h in solution, then wash in 0.5% acidified water
shortly). The background of images was all red under ordinary
microscopy, but not yellow as expected, collagen can be seen barely with
dark red. I extended washing time in acidified water, but no improvement
was gotten. I just wonder any one had similar experience and how to
improve it?

Thanks in advance.

Dr. Xilong Li
Hypertension Division, Internal Medicine
University of Texas Southwestern Medical Center 
5323 Harry Hiness Blvd-J4.142
Dallas, TX 75390

Tel: 214-648-9966(L)
Fax: 214-648-7902

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