[Histonet] VEGF antibody, clone VG1
Has anybody successfully used Dako's mouse-anti human VEGF, clone VG1? We
have tried it at 2-4 ug/ml with 7 different pretreatments (enzymes, HIER
with different buffers) on various tissues (including tonsil, colon,
pancreas, breast carcinoma, endometrium) and the best we have got are a few
isolated positive exocrine cells in the pancreas and the lumina of vessels
that stain positive. This is in sharp contrast to what Turley et al. (J
Pathol 186:313-18, 1998), who developped the antibody, describe.
Visualization is with a conventional 3-step ABC-system. Anybody with better
results with that particular clone? Pretreatment? Antibody concentration?
Positve controls? Many thanks for your feedback.
Institute of Pathology, University of Bern, Switzerland
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