Hi...
I've been a histotech in Florida for years and have purchased quite a number of reconditioned/refurbished instruments from Belair Instruments...there also happen to be the only remanufacturer of Histology laboratory equipment that comes with a full one year unconditional guarantee...when you see their instruments, you'd swear they were brand new!!  The number for Belair is: 973-912-8900.  Ask for Mike McDougal. He is the national sales manager and a pleasure to do business with....
 
Steven Lee HTASCP 
 
 
-----Original Message-----
From: histonet-request@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Sent: Fri, 18 Aug 2006 1:01 PM
Subject: Histonet Digest, Vol 33, Issue 23


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Today's Topics:

   1. RE: Collagen testing (Liz Chlipala)
   2. purchasing refurbished equipment (Rice, Michael)
   3. Shirley is away : 18 August 2006 (Friday) (Shirley PHUA)
   4. Re: Histonet Digest, Vol 33, Issue 22 (Steven2146@aol.com)
   5. mouse eyes (Sally Prouty)
   6. CAP Inspection question for Proficiency Testing in    Histology
      (Amy Porter)
   7. Re: CAP Inspection question for Proficiency Testing in
      Histology (Rene J Buesa)
   8. Pascal (Orr, Rebecca)
   9. Ultra-Low Freezer Recommendation (Amber Tyler ANTYLER)
  10. Salary in Texas (Reuel Cornelia)
  11. Refurbished Equipment (Kelly Larson)
  12. reply to Mouse Eyes (Lawrence D Lanberg/O/VCU)
  13. Why tissue sections from resin blocks have black  deposits?
      (Sulekha Ravi)
  14. Alizarin red staining  (pex)
  15. Muscle ATPase: Barbital Substitute (Michele French)
  16. Mouse eyes (Paula Pierce)


----------------------------------------------------------------------

Message: 1
Date: Thu, 17 Aug 2006 11:10:29 -0600
From: "Liz Chlipala" 
Subject: RE: [Histonet] Collagen testing
To: "'Sebree Linda A.'" , "'Histonet'"
    
Message-ID: <000001c6c220$0a735370$0300a8c0@domain.Premier>
Content-Type: text/plain;   charset="US-ASCII"

Linda

We can run Collagen I, II, III, IV and VII for immunohistochemistry, We are
currently working on Collagen X, we also can run aggrecan and then markers
for dermis specimens such as talin, vitronectin, etc.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda
A.
Sent: Thursday, August 17, 2006 10:50 AM
To: Histonet
Subject: [Histonet] Collagen testing


> Hello histonetters,
> 
> A researcher at our institution is looking for a reference lab that
> performs collagen testing by IHC.  I know of Collagen Types I, II, III
> & IV but don't know if there are any others.  If there are reference
> labs out there that perform more than just Type IV, please let me know
> and I'll pass the information along.
> 
> Thank you,
> 
> Linda Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-3224
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
> 
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------------------------------

Message: 2
Date: Thu, 17 Aug 2006 13:38:37 -0400
From: "Rice, Michael" 
Subject: [Histonet] purchasing refurbished equipment
To: 
Message-ID:
    <3BC92F29BE821745AB15E04C98EE028D6D3964@HCH2KMAIL.holy-cross.com>
Content-Type: text/plain;   charset="us-ascii"

The main idea, is to find and use a company that will guarantee its
equipment, and will furnish a list of references. I have used Imeb on
several occasions for refurbished  items and have always been satisfied
with their level of service.

 

Michael Rice CT.HT(ASCP)

Supervisor Of Pathology

Holy Cross Hospital

Ft Lauderdale, Fl 33308

954.776.3070

 



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------------------------------

Message: 3
Date: Fri, 18 Aug 2006 02:06:13 +0800
From: Shirley PHUA 
Subject: [Histonet] Shirley is away : 18 August 2006 (Friday)
To: histonet 
Message-ID:
    
    
Content-Type: text/plain; charset=US-ASCII

I will be out of the office from  08/18/2006 to 08/18/2006.

 I will return on 21 August 2006 (Monday).

Pathologists : I will process your requests when I return. Otherwise, if
urgent, please forward your mail to henry_kyaw@hsa.gov.sg

Regrets for the inconveniences caused.




------------------------------

Message: 4
Date: Thu, 17 Aug 2006 14:26:46 EDT
From: Steven2146@aol.com
Subject: [Histonet] Re: Histonet Digest, Vol 33, Issue 22
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset="US-ASCII"

HI...
If you're looking for reliable refurbished histology equipment, Belair is  
the best out there....the machines not only are TOTALLY reconditioned, but when  

you receive them, they look brand new....Belair, as far as I know, is the 
only  company out there selling refurbished equipment, that offers a one year,  
unconditional guarantee.
 
Steven Lee HT ASCP


------------------------------

Message: 5
Date: Thu, 17 Aug 2006 14:13:14 -0500
From: "Sally Prouty" 
Subject: [Histonet] mouse eyes
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format=flowed


Does anyone have a protocol for processing mouse eyes that doesn't cause 
them to end up looking like raisins? Besides hand processing we haven't find=20
a consistant method.

Sally J. Prouty
Research Assistant, Campbell Laboratory
Howard Hughes Medical Institute
Department of Physiology & Biophysics
University of Iowa College of Medicine
4283 Carver Biomedical Research Building
Iowa City, IA  52242-1101

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------------------------------

Message: 6
Date: Thu, 17 Aug 2006 15:23:21 -0400
From: "Amy Porter" 
Subject: [Histonet] CAP Inspection question for Proficiency Testing in
    Histology
To: 
Message-ID: <002301c6c232$99d82330$8e7a0923@HistoJJ>
Content-Type: text/plain;   charset="iso-8859-1"

Can anyone out there is histoland tell me how they are meeting the proficiency 
testing program requirements for CAP inspections specifically with regards to 
histology..... I have spoken with CAP about the HistoQIP program and they 
consider that an educational program, not a proficiency program.  Just wondering 
what other options are out there if any.  Thanks for your responses in advance!

Amy S. Porter, HT(ASCP) QIHC - Supervisor
Michigan State University
Investigative HistoPathology Laboratory     
Department of Physiology / Division of Human Pathology
2100 Biomedical Physical Sciences Bldg. Room #2133
East Lansing, MI  48824-3320
Phone:  (517) 355-6475 ext 1480 / Fax:  (517) 432-1368
Email:  portera@msu.edu
www.humanpathology.msu.edu



------------------------------

Message: 7
Date: Thu, 17 Aug 2006 12:40:46 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] CAP Inspection question for Proficiency
    Testing in  Histology
To: Amy Porter , histonet@pathology.swmed.edu
Message-ID: <20060817194046.17625.qmail@web61216.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Amy:
  I used to have "competency check lists" and "standard of performance 
descriptions" for each mayor task. Along the year I used to observe the activity 
of each HT in each task and evaluated them against those lists.
  The deficiencies I found were documented and used for the individual PI of=20
each HT, which went to their personal file.
  When during the CAP inspections I was asked about this aspect I showed them 
the PI developed for each HT that required a re-training on any given task.
  They were pleased with this approach to documenting the proficiency in 
completing histology tasks.
  Hope this will help you!
  René J.

Amy Porter  wrote:
  Can anyone out there is histoland tell me how they are meeting the proficiency 
testing program requirements for CAP inspections specifically with regards to 
histology..... I have spoken with CAP about the HistoQIP program and they 
consider that an educational program, not a proficiency program. Just wondering 
what other options are out there if any. Thanks for your responses in advance!

Amy S. Porter, HT(ASCP) QIHC - Supervisor
Michigan State University
Investigative HistoPathology Laboratory 
Department of Physiology / Division of Human Pathology
2100 Biomedical Physical Sciences Bldg. Room #2133
East Lansing, MI 48824-3320
Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368
Email: portera@msu.edu
www.humanpathology.msu.edu

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http://lists.utsouthwestern.edu/mailman/listinfo/histonet




            
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------------------------------

Message: 8
Date: Thu, 17 Aug 2006 15:01:09 -0500
From: "Orr, Rebecca" 
Subject: [Histonet] Pascal
To: 
Message-ID:
    
Content-Type: text/plain;   charset="us-ascii"

We use the Biocare Decloaker in our lab.  It's the same instrument as
the Pascal. We use Dako ER/PR on our lab.
We can run 4 tissue tek slide rack/containers at a time for a total of
98 slides in one run.

Hope this helps.

Becky Orr, CLA, HT(ASCP) QIHC
Assistant Manager, Anatomic Pathology
Evanston Northwestern Healthcare
847-570-2763


Hello All,
 
  I was wondering if anyone can tell me if they are using Pascal (from 
Dako) for ER/PR? If so, how do you like it? Does it take longer than a 
steamer for antigen retrieval? What do you think of it? Any information 
you can provide would be great! 
 
  Thanks!!
 
  Jessica Piche-Grocki, HT(ASCP)
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------------------------------

*****************



------------------------------

Message: 9
Date: Thu, 17 Aug 2006 16:28:59 -0400
From: Amber Tyler ANTYLER 
Subject: [Histonet] Ultra-Low Freezer Recommendation
To: histonet@lists.utsouthwestern.edu
Message-ID:
    
Content-Type: text/plain; charset="US-ASCII"

Hello, 

Has anyone had any experience with ultra-low freezers by Sanyo. I already 
know about rev-co, but I found a comparable freezer by sanyo that is a bit 
cheaper. 

Thank you, 
Amber


Amber N. Tyler, Ph.D.
Research Scientist
Dept of Psychology (PO Box 26170)
296 Eberhart Building, Walker Avenue
University of North Carolina - Greensboro
Greensboro, NC 27402





------------------------------

Message: 10
Date: Thu, 17 Aug 2006 16:44:10 -0500
From: "Reuel Cornelia" 
Subject: [Histonet] Salary in Texas
To: 
Message-ID: 
Content-Type: text/plain; charset=US-ASCII

Just wanted to hear some feedback on latest Wage data for Histotech
supervisors particularly in dallas,texas. I want specifics, either per
hour or salaried.Thank you.
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------------------------------

Message: 11
Date: Thu, 17 Aug 2006 18:02:22 -0400
From: "Kelly Larson" 
Subject: [Histonet] Refurbished Equipment
To: histonet@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; format="flowed"


   We  purchased  a  used  Tissue-Tek processor from Rankin.  It had some
   problems  to  start  with but they stood by their product and fixed it
   for  us  free of charge.  They seem to be pretty honest people to work
   with.


------------------------------

Message: 12
Date: Thu, 17 Aug 2006 23:18:09 -0400
From: Lawrence D Lanberg/O/VCU 
Subject: [Histonet] reply to Mouse Eyes
To: prouty27 <%t@vcu.edu> msn.com
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
    
Content-Type: text/plain; charset="ISO-8859-1"


   Dear Ms. Prouty,



   I  do  mouse  eyes  -- ev   looking  like  raisins,  do  you  me   
wrinkle????  Or  are  you  simply referring th   Osmium Tetroxide imparts?



   I  am always developing our protocols, but my ultra-thin sections come
   out     more specifi
   Larry Lanberg
   PO   Sanger Hall 2-032
   Richmond, VA&n   804.828.7974
   804.648.3246

   

------------------------------

Message: 13
Date: Fri, 18 Aug 2006 10:19:30 +0100 (BST)
From: Sulekha Ravi 
Subject: [Histonet] Why tissue sections from resin blocks have black
    deposits?
To: histonet@lists.utsouthwestern.edu
Message-ID: <20060818091930.79205.qmail@web25009.mail.ukl.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Dear members
  I have a problem with my resin blocks.We make resin blocks for non decalcified 
bone and vessel with metallic stent in my research laboratory. We dehydrate the 
tissue in ascending grades of alcohol, impregnate with methyl methacrylate 
monomer and then make blocks in polymerised methyl methacrylate.Some areas of 
tissue in these blocks have white opaque precipitate like dirt in it. When 
sections from such blocks are seen under microscope, these areas are black 
precipitate like deposits, masking the entire cellular architecture. We tried 
sonicating the sections in water with and without detergents. But nothing 
helped. Please help me to rectify this serious problem.
  Thanks
  Sulekha.
  Sr. Technical Asst
  SCTIMST
  Trivandrum
  Kerala
  India 695012

        
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------------------------------

Message: 14
Date: Fri, 18 Aug 2006 18:55:32 +0800 (CST)
From: pex 
Subject: [Histonet] Alizarin red staining 
To: Histonet@lists.utsouthwestern.edu
Message-ID: <20060818105532.40790.qmail@web15212.mail.cnb.yahoo.com>
Content-Type: text/plain; charset=gb2312

Hello,
   
  I would like to perform Alizarin Red staining for mineralization nodules.
  Can someone do me a favor to show your protocol about it ?
   
  Thank you.
   
  Guofeng 

        
---------------------------------
 ÑÅ»¢Ãâ·ÑÓÊÏä-3.5GÈÝÁ¿£¬20M¸½¼þ

------------------------------

Message: 15
Date: Fri, 18 Aug 2006 09:50:35 -0400
From: Michele French 
Subject: [Histonet] Muscle ATPase: Barbital Substitute
To: histonet@lists.utsouthwestern.edu
Message-ID: <44E5C5AB.4070702@bms.com>
Content-Type: text/plain; format=flowed; charset=ISO-8859-1

Good Morning Everyone!
    I searched the archives and saw a few messages back in 2004 about 
using Glycine as a substitute for Sodium Barbital in the ATPase Stain 
for muscle fiber typing. The original e-mail did not include a recipe 
for the replacement of the barbital acetate preincubation solutions. It 
only talked about one preincubation solution at pH 9.4. I was wondering 
if anyone currently uses this substitute successfully and could send me 
your procedure?
Thanks!

Michele French
Bristol-Myers Squibb
Lawreceville, NJ



------------------------------

Message: 16
Date: Fri, 18 Aug 2006 07:49:57 -0700 (PDT)
From: Paula Pierce 
Subject: [Histonet] Mouse eyes
To: histonet@lists.utsouthwestern.edu
Message-ID: <20060818144958.28339.qmail@web50107.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I process to paraffin and cut whole eyes from everything from drosophila to 
ostrich with no retinal detachment or shattered lenses.
   
  How are you processing now?


Paula Pierce, HTL(ASCP)HT

Excalibur Pathology, Inc.
630 N. Broadway
Moore, OK 73160
405-759-3953
contact@excaliburpathology.com
www.excaliburpathology.com

------------------------------

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End of Histonet Digest, Vol 33, Issue 23
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