We have tried Zymed's mouse-anti-MUC4, clone 1G8, on a series of colon 
carcinoma at 1 ug/ml, 7 different pretreatments (enzymes, HIER with various 
buffers), visualization with a conventional 3-step ABC-system. After HIER we 
get positive staining of endothelial cells (which, according to the 
manufacurer, is normal), however - in contrast to what the datasheet says - 
we get no or almost no staining of epithelial cells / carcinoma cells. Has 
anybody been able to demonstrate MUC4 in colon carcinoma? Any suggestions 
for better positive controls (okay, the endothelia are positive, but not the 
tumors), pretreatment, antibody concentration? Many thanks!

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland 


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