[Histonet] Frozen tissue storage

From:"Osborn, Sharon"

    There are systems you can purchase to store tissues or OCT blocks in
an orderly fashion in -80 freezers.  We place individual blocks in
Fisher Twirl 'Em sterile sampling bags 3x5 inches, label and place in a
correctly sized cardboard box that then fits into stainless shelves.
These shelves hold 12 boxes each stacked in 4 rows of 3 boxes each.
Four shelves will fit onto one shelf of a -80.  The Freezer is numbered,
the boxes are numbered and the racks are numbered ie.. 5/10/48 would
interpret as freezer #5, rack # 10 and Box #48. This is an example of
what you could set up for storing your tissues.  
    As Rene mentioned,tissues will keep as long as they are properly
preserved, sealed and the -80 freezers do not go down; or when the
freezer goes down, someone rescues its contents BEFORE everything
melts--those emergency contacts again!  I also have cut frozen sections
from tissues that have been frozen for years.  I have also cut paraffin
sections from blocks over 20 years old that cut well when properly
stored.  I have also had some that the bugs had dinner from so there was
no tssue left! 

Okay, Joe, your turn! :-)

Sharon Osborn
DNAX, SPBioPharma
Palo Alto, CA

Mathew DeGutes  wrote:
  I have a couple questions. In the lab I work in we have started to
accumulate a number of experiments worth of tissue blocks that we would
like to keep for reference at later dates. Generally tissue survives
better while still embedded in a block than when in slide form, so I was
curious as to what sorts of methods others here employ to keep order and
good preservation of blocks in -80s? Up until now we have been just
keeping individual sets of blocks in Ziplock bags, but it has gotten to
be unwieldy. I am looking for any suggestions of a good way to keep the
blocks well preserved and easily recovered in -80s.
This message and any attachments are solely for the
intended recipient. If you are not the intended recipient,
disclosure, copying, use or distribution of the information 
included in this message is prohibited -- Please 
immediately and permanently delete.
Histonet mailing list

<< Previous Message | Next Message >>