[Histonet] Ethanol fixation

From:Xiao-Qun Zhang

Hello All,

I am new for the histonet. I am sorry If my question has already been 
discussed many times.
My question is if E14.5 mouse embryos fixed over night in 4% PFA in 
the cold room and then kept in 70 % ethanol for 4 days in the cold 
room will still be ok for sectioning and in situ hybridization?
Any comments are highly appreciated!

Xiaoqun Zhang
Xiao-Qun Zhang, PhD
Dept. of Medical Biochemistry and Microbiology
Box 582, Biomedical Center
S-751 23 Uppsala, Sweden

Phone: +46 (0)18 471 4260
Fax: +46 (0)18 471 42 44
Email: Xiao-Qun.Zhang@imbim.uu.se

Histonet mailing list

<< Previous Message | Next Message >>