[Histonet] Deparaffinization Times
You could also check after immersion in the first alcohol. The tissues that
are clear of paraffin become more opaque while you will see spots/patches
that look different if all the wax isn't out. This can be another indicator of
sufficient time in Xylene. When I worked with paraffin sections years ago,
we would do at least 3-5 minutes in 3 separate xylene baths. 1 minute in 2
xylenes sounds a bit short....but may ultimately depend on the type and
thickness of tissue.
Albert C. Grobe, PhD
International Heart Institute, Tissue Engineering Lab
Saint Patrick Hospital
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