[Histonet] Deparaffinization Times


You could also check after immersion in the first alcohol.  The  tissues that 
are clear of paraffin become more opaque while you will see  spots/patches 
that look different if all the wax isn't out.  This can be  another indicator of 
sufficient time in Xylene.  When I worked with  paraffin sections years ago, 
we would do at least 3-5 minutes in 3 separate  xylene baths.  1 minute in 2 
xylenes sounds a bit short....but may  ultimately depend on the type and 
thickness of tissue.  

Albert C.  Grobe, PhD
International Heart Institute, Tissue Engineering Lab
Saint  Patrick Hospital

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