Re: [Histonet] liver albumin

From:Geoff McAuliffe

Hi Steve:

    Albumin is a very large protein that is largely confined to the=20
vascular system. Yes, it is made in hepatocytes but I suspect that it=20
diffuses quickly in sections. Also, the form that exists inside cells=20
may not react with your marker? I suspect that freeze drying or freeze 
substitution might (emphasis on might) be the way to go here. Also, a=20
PubMed or Medline search will reach a much larger database than Histonet 
and you might find the methods you seek.

Geoff


Steven Coakley wrote:

>Good afternoon,
>  We are attempting to stain cyrosections of mouse liver for albumin.  We have currently tried both unfixed and fixed/sucrose sectioned postfixed in 4% Formalin/PBS.  Vascular stains but otherwise nothing.  Is anyone doing anything like this?
>   
>  Thanks,
>   
>  Steve
>
> 		
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff@umdnj.edu
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