Re: [Histonet] liver albumin
Hi Steve:
Albumin is a very large protein that is largely confined to the=20
vascular system. Yes, it is made in hepatocytes but I suspect that it=20
diffuses quickly in sections. Also, the form that exists inside cells=20
may not react with your marker? I suspect that freeze drying or freeze
substitution might (emphasis on might) be the way to go here. Also, a=20
PubMed or Medline search will reach a much larger database than Histonet
and you might find the methods you seek.
Geoff
Steven Coakley wrote:
>Good afternoon,
> We are attempting to stain cyrosections of mouse liver for albumin. We have currently tried both unfixed and fixed/sucrose sectioned postfixed in 4% Formalin/PBS. Vascular stains but otherwise nothing. Is anyone doing anything like this?
>
> Thanks,
>
> Steve
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff@umdnj.edu
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