Re: [Histonet] Ventana PanCK-Cross reacts with GFAP

From:"Richard Cartun"

Cross-reactivity of GFAP with low molecular weight cytokeratin
antibodies (e.g., CAM5.2 and others) has been well documented over the
years.  A sensitive, but epithelial-specific (does not cross-react with
GFAP)  cytokeratin monoclonal is OSCAR (developed by Dr. Allen Gown)
available from Signet Laboratories.


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax

>>> "Richard Hessler, M.D."  08/09/06 4:33 PM
This cocktail seems to be a mix of AE1/AE3 and one that I am not
familiar with-PCK26. After years of Dako I am now using Ventana for
first time and was shocked to see 4+ staining of reactive gliosis with
this Cocktail. Has anyone seen this? Why do they not use just AE1/AE3
like everyone else?
But, most importantly, how is this stuff "approved for in vitro
diagnostic use"???
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