Re: [Histonet] RE: pfa vs. formalin
Hi Paul,
Actually, commercial formaldehyde solutions may contain anywhere from 5-15%
methanol.
It often depends on geographic location and what time of year the reagent is
shipped.
Over the years, I have performed many side-by-side studies of 4% phosphate
buffered formaldehydes,
prepared from both commercial formaldehyde solutions and from
paraformaldehyde.
The fixatives were used for a wide range of investigations including
morphology using routine oversight and many special stains,
histochemistry, IHC, ISH and EM.
In NO case could I or anyone else detect any difference
Are you aware of any definitive studies which prove that the presence of
0.5-1.5% methanol is deleterious for any investigation?
Regards,
Bryan
----- Original Message -----
From: "Monfils, Paul"
To:
Sent: Friday, August 04, 2006 2:36 PM
Subject: RE: [Histonet] RE: pfa vs. formalin
> The only difference is that commercial formaldehyde solution contains a
> stabilizer, usually 10 to 15% methanol. Therefore, 10% NBF made up from
> such a solution contains 1 to 1.5% methanol, while 10% buffered NBP
> contains
> nothing but water, formaldehyde, and buffer salts. For routine
> morphological studies this makes no difference whatsoever. But for some
> special studies the presence of a small amount of methanol could be
> deleterious, and for any such procedures paraformaldehyde would be
> preferable.
>
>
>
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