Michele,

I have successfully used Novocastra's monoclonal CD34 antibody for
endothelial cells  on human FFPE tissue. It requires trypsin digestion
rather than HIER. 
Hermina

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannah,
Michele F.
Sent: Wednesday, August 02, 2006 1:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] endothelial cell antibody

Has anyone worked with an antibody to endothelial cells that has worked
well for them?   If appropriate, was it successful in lung tissue and/or
what were the conditions (FFPE, frozen, ect)?  I am looking at several
different possibilities and wanted to know what other people were using.
Thanks!

Michele

Michele F. Hannah  M.S.
Department of Molecular Microbiology & Immunology Johns Hopkins
Bloomberg School of Public Health
615 North Wolfe Street  Room  E3201
Baltimore, Maryland 21205
Phone: (410) 614-7794
Fax:     (410) 955-0105
 

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