[Histonet] autostainers & TMA's

From:"Garry Ashton"

Dear histonetters
I have 2 topics up for discussion.
 
Firstly immunostainers. 
We are a small research lab and hence I'm interested in a machine that
is totally flexible with regards to running several antibodies with 2 /3
different methods.
Ideally an "open system" that will  also dewax & retrieve if possible.
We've looked at dako and biogenix which are both open  systems and
ventana and vision biosytems.
Any comments on the above, or on any other systems available would be
great.
I know this subject ha been covered several times on the histonet, but
an up to date view would be much appreciated.
 
Secondly has anybody had any problems with regards to TMA sections
falling off slides.
We use 1mm cores and only put about 100 in each block, which are then
cut and placed on APES coated slides.
Sometimes up to 30% of the core may come off.
I think next we'll try charged slides. Does anybody have any opinions /
ideas on this
 
Many thanks in advance for any opinions.
 
 
 
 
 
Garry Ashton
PICR
UK
 
--------------------------------------------------------

 
This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible.
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>