[Histonet] Re: Setting Ink
Karen Heckford asks >>Does anyone know if there is something that sets the
on tissue after you have inked the margins?<< and Patsy Ruegg (along with
several others) replies >>We use Bouin's fixative to set ink, just dip it in for
a few seconds then go on your way.<<
Bouin's fixative contains picric acid, a potential explosion hazard that can
give you trouble with regulatory agencies, besides being toxic, and ruinous to
clothing (particularly since it is inclined to eat through latex).
Acetone, also often recommended, is a fire and explosion hazard.
If 2% to 3% acetic acid works - and I haven't tried it personally - why not
If you blot the specimen dry adequately, the ink will usually stick to it -
India ink, Davidson and similar marking inks, and tattoo inks - I've used them
all. Ink won't stick to cauterized surfaces (such as LEEP specimens), but the
pathologist can see these margins under the microscope whether they're inked
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