[Histonet] Long term storage of mouse brains

From:Amber Tyler ANTYLER


I'm just beginning to learn histochemical techniques. I will have a number 
of adult mouse brains I would like to store for about a year. What is the 
best way to do this without causing tissue damage?

As of right now, I was thinking of fixing in 2-methyl-butane/dry ice then 
storing at -80C. 

I would like to eventually do Autoradiography and/or Immunohistochemistry 
staining in the hypothalamus and hippocampus. 

Thank you for any advice and information you can provide. 

Amber N. Tyler, Ph.D.
Research Scientist
University of North Carolina - Greensboro
Greensboro, NC 27402
Office: 336.334.4046

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