Michele,
I have used an anti-VWF on paraffin and cryostat sections as well as eNOS  
abs.  The VWF was a polyclonal, and consequently had a bit more background,  but 
the ECs were stained very nicely.  The argument for the VWF, is that  not all 
ECs may express VWF.  eNOS should be present in ECs. 
Albert  



Albert C.  Grobe, PhD
International Heart Institute, Tissue Engineering Lab
Saint  Patrick Hospital
554 W. Broadway
Missoula, MT 59802

(406) 329-5634  Lab
(406) 329-5880 Fax
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