[Histonet] Cytoarchitectural analysis of axons and thick sectioning of neural tissue
Dear histonet professionals,
My PI and I have been accumulating a large bank of 40 human brains and
spinal cords with ALS and controls with non-neurologic conditions.
Certain areas of the brain and the entire cord has either been fixed in
10% NBF and then processed through to paraffin or frozen for future
molecular studies. My PI has been reading a number of papers, and
noticed that usually the brains are sectioned very thick (20+ microns).
I believe that I understand why, but I have tried to section our samples
that thick, and I have had a lot of problems. Would it be easier with a
softer paraffin, (I am using Tissue-Tek's VIP brand), a different
microtome (maybe a sliding, or a vibratome) or some other method I
Secondly, he wants to embed some of our spinal cord specimens in Epon
for cytoarchitectural analysis of the spinal tracts. Typical procedures
I have seen require the tissue to be fixed in gluteraldehyde, mine are
fixed in formalin. Are the two methods interchangeable, or could I
postfix in gluteraldehyde and then embed in epon?
Thanks in advance for any tips.
Patrick Laurie, HT (ASCP)
Benaroya Research Institute
1201 9th Ave
Seattle, Wa 98101
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