Re: [Histonet] sentinel biopsies
We receive the nodes fresh. Bisection if the thickness is less than 0,6 cm.
If more, we try to trim into three (or rarely four) slices. Than, prepare 2
slides from each surface. One imprint and one scrape, which are rapid H&E or
MGG-quick stained. Than submit each slice in seperate cassettes. We do not
concern about radiation issue and process as routine. We cut the the block
without any trimming to avoid any tisue loss. Serial sections 50 microns
apart are done, till the end of the tissue. This method usually produces a
lot of slides. However one can be sure that, no micrometastasis is left
behind in the block. If primary tumor diagnosis is lobular carcinoma, each
serial section might be accompanied by one unstained positively charged
slide for probable IHC.
According to our experience (more than 21.000 surgicals and about 25.000
cytology cases annually) , frozen sectioning, does not give any additonal
information compared to cytology slides and the risk of tissue loss or
suboptimal morphology due to freezing artefacts is a real threat.
U. Ince MD
----- Original Message -----
From: "Satterfield, Marirose"
Sent: Wednesday, August 31, 2005 1:32 PM
Subject: [Histonet] sentinel biopsies
>I would like to know how everyone is handling these biopsies. Extremely
> interested in if you facility does frozen sections or touch prints and
> special handling (if any) you do. Our pathologists are discussing these
> options with a surgeon and we would like to know how everyone else is
> handling these specimens. I cannot find any CAP guidelines. Any input
> be appreciated.
> M. Satterfield
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