Re: [Histonet] (no subject)

From:Lori Richey

We fix all precut control tissue  slides in cold acetone for 5 
minutes,and allow them to air dry before storing them in the -70. 

Matt Prideaux wrote:

>Dear Histonetters,
>A colleague wishes to store cytospun cell preparations in the freezer before
>doing immunocytochemistry for CD31 and CD44. She would like to know whether
>it's better to fix the slides before freezing or after thawing them prior to
>staining. Also, which fixatives would you recommend? 

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