Re: [Histonet] mitochondrial antibody enquiry and DAB waste disposal

From:"Andi Kappeler"

Hi Megan

mo-a-Mitochondrial Antigen, clone 113-1, BioGenex CatNo MU213-UC, works 
reasonably well on FFPE tissue. We had best results after HIER (citrate) in 
a pressure cooker and with the mAb diluted at 1:50 (which is a relatively 
useless information, however BioGenex is also one of those companies that do 
not specify Ig content in ug/ml for their antibodies, therefore I have to 
give you the information '1:50' rather than x ug Ig/ml). Hope this helps

Andi Kappeler
Institute of Pathology, University of Bern, Switzerland

----- Original Message ----- 
From: "Megan Clarke" 
Sent: Wednesday, August 17, 2005 10:23 AM
Subject: [Histonet] mitochondrial antibody enquiry and DAB waste disposal

> Hi. I have 2 queries.
> Firstly, could anyone please assist me in finding a supplier for anti- 
> mitochondrial antibody for use in FFPET (formalin fixed paraffin embedded 
> tisue) and
> secondly, with regard to DAB waste disposal, is the potassium 
> permanganate/sulphuric acid  method still the one of choice for 
> neutralizing DAB or is there some new magic available?  If their are any 
> BONDMAX users out there, I would appreciate it  if you could share your 
> DAB waste disposal method with me.
> Thanking you
> Z Haffajee/Megan Clarke
> IHC unit
> Newcastle
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