Re: [Histonet] blood sample for TEM...

From:Ul Soo Choi

hi! dear histonetters,
thank a lot for your kindness in all your quick, and detailed replies.
Everytime I rely on you, I am greatly benefited. Thanks again.

Ul Soo

On 8/23/05, Home  wrote:
> Hello Ul Soo Choi,
> What you can do is, spin down your heparinized blood cells (unfixed).
> What you get is a buffycoat (white cells) on top of your erythrocytes.
> Gently remove the serum and then ad gently your fixative (2% glutaraldehyde/
> buffer) without disturbing the buffycoat layer, let it stand for at least 3
> hours on ice and then you can remove the  buffycoat with your white cells as
> a compact layer. You can cut it in pieces and proces it like you would
> normally do with tissue samples.
> Huib
> ----- Original Message -----
> From: "Ul Soo Choi" 
> To: 
> Sent: Monday, August 22, 2005 6:03 PM
> Subject: [Histonet] blood sample for TEM...
> Hi histonetters!
> I am interested and in need of a protocol for preparing blood samples
> for transmission electron microscopy. But I am short of appropriate
> protocol for fixation, and processing for it.
> I would like to see cat neutrophils and lymphocytes by TEM, and
> Would anyone there who has experiences in blood specimen for TEM, kindly
> give me some tips for preparing blood samples for EM?
> Thank you for any inputs. I would appreciate.
> Best regards,
> Ul Soo Choi
> DVM., PhD.
> Dept of clinical pathology,
> CVM, SNU, Seoul, Korea
> 82-02-880-8688
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