Re: [Histonet] aldehyde fuchsin

From:"John A. Kiernan"

The solution of aldehyde fuchsine has a rather
short shelf-life (8 weeks) so it's best to make 
up your own. It is also possible to recover
the aldehyde fuchsine as a precipitate that can 
be collected by filtering, dried and stored
for 10 or more years. Solutions made from aldehyde
fuchsine powder (0.125% in 70% ethanol with 
1% acetic acid) are more stable than the traditional 
directly made solution, being good for about 2 years. 

It is said that solutions of aldehyde fuchsine
made from the powder will not stain pancreatic
islet B cells without prior permanganate oxidation 
(see Mowry,RW 1978 Stain Technol. 53: 141-154. This
paper also has useful tips for other uses of
aldehyde fuchsine. 
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Robin Newlin wrote:
> Hi All
> Does anyone know if aldehyde fuchsin is available commercially, or do I have to brew my own.
> Thanks
> Robbin Newlin
> Manager, Cell Imaging/Histology Core Facility
> The Burnham Institute
> 10901 N. Torrey Pines Road
> LaJolla, Ca. 92037
> 858-646-3100 ext.3552
> _______________________________________________
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