Re: [Histonet] PAS-F

From:Gayle Callis

Easy, simply replace the periodic acid with 4% chromic acid (chromium 
trioxide is the dry chemical).  This is the same reagent used in a GMS 
stain, and you do the same timing for treatment of section, rinse as you 
would for GMS then proceed to Schiffs reagent.   Do not drain dump Chromic 
acid, it should be collected due to chromium ions.  WE only use our chromic 
acid for a week, the dispose of it.

  At 09:26 AM 8/23/2005, you wrote:
>I am sure someone has this procedure.  I have seen it somewhere , but can
>not find it.  I need a PAS-F using Chromic Acid instead of Periodic Acid.
>Hope you can help.  I am not talking about the Gridley stain.  Thanks for
>you help .
>Rita Riddle HT(ASCP)PA
>Lead Histology Tech
>Lexington Medical Center
>West Columbia, SC
>Telephone 803-791-2881
>Fax  803-791-2331
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Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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