Re: [Histonet] Michel's medium for skin biopsies

From:"Robyn Vazquez"

I believe Michels is a transport media, not a fixative media.  You don't want the tissue fixed, if you are doing antibody testing on it, in the fresh tissue sense.  I use Zeus Tissue Transport Media, my doctor seems to like it better.


>>> "Atkinson Paul B"  8/24/2005 2:18 AM >>>

We are considering using Michels's transport medium for skin biopsies in our
laboratory. Have other laboratories found its use worthwhile? The original
1973 paper only mentions the effect on immunoglobulin staining, is it also
effective in preserving complement antigenicity? Also how long does the
tissue need to be in the fluid for the 'fixation' to occur?

Many thanks

Paul Atkinson

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